Physico-chemical and bio-chemical aspects of the reservoir water has been investigated to assess the quality of water for fish growth and survival. The variations of the physico-chemical properties of water samples directly influence the biotic communities and primary productivity of the water body of Barnoo reservoir. The aim of the present study was to determine the physical, chemical and biological characteristics and to characterize the fish growth in Barnoo reservoir. Temperature, Water transparency, pH, Dissolved oxygen, Free CO , Alkalinity, TDS, 2 Hardness, Calcium, Conductivity, Gross primary productivity are the parameters were measured and indicates that the reservoir is medium productive. A total number of 30-genera of zooplanktons viz. Rotifera, Cladocera, Copepoda and 61 genera of phytoplanktons viz. Basillariophyceae (Diatoms), Chlorophyceae, Cyanophyceae and Dinophyceae were observed and recorded. Growth performance of stocked fishes i.e. Catla, Rohu and Mrigala fishes was observed and found to be encouraging and satisfactory. In our opinion it was due to proper selection of fish species, size and number of fingerlings stocked and water quality of the reservoir favors stocked fish for better survival.