Seeds of Syzygium cumini (L.) Skeels of family Myrtaceae are reported in Ayurveda - Unani and Siddha medicinal system to have great medicinal value. All parts of the Jamun used to cure various health diseases like digestive disorders, liver problems, diabetes, dental problems and also skin ailments. The present communication provides a detailed account of the pharmacognostic evaluation carried out on Jamun seeds. The study includes macro and microscopic characters, preliminary phytochemical analysis, fluorescence study, physicochemical parameters and High Performance Thin Layer Chromatography (HPTLC) fingerprinting aspects. Established parameters can be used as standards for quality control and identification of the plants in compound formulations and also preparations of a monograph of the plant.