Malathion is one of the pesticides which is commonly used in Agriculture in India. In this study, microbial strains effective in degrading Malathion were isolated from the soil samples treated with Malathion. Isolated bacterial strains were exposed to 10 mg/l of technical grade Malathion to determine the degradation efficiency. Significant degradation of Malathion i.e. 43% by C1 and 40% by C2, was observed in 10th days under laboratory conditions. C1 was identified as Staphylococcus arlettae and C2 as Flexivirga using 16S rRNA technique.