Year: 2017, Issue: December

Bioinsecticidal effect of endophytic fungal extract of Azadirachta indica against Callosobruchus chinensis Linn. (Coleoptera : Bruchidae).

Author:

P. N. Jadhav and A. B. Pardeshi

Keyword:

Callosobruchus chinensis, Endophytic fungi, Mortality, Bioinsecticide, Azadirachta indica.

Endophytic fungi are microorganisms that live inside the plant without causing any over negative effect on plant tissues; rather they protect the host plant from pests and diseases. The insecticidal activity of endophytic fungal extract from leaf and seed of Azadirachta indica against pulse beetle, Callosobruchus chinensis, were studied. Endophytic fungi were isolated from the leaf and seed of Azadirachta indica. The various concentrations of methanol and ethyl acetate crude endophytic fungal extracts were tested against Callosobruchus chinensis. The percent mortality was recorded after 96h. The insecticidal activity of the endophytic fungi isolated from leaf of Azadirachta indica were (LD10 =19.81 mg/kg, LD50 =84.63 mg/kg) in methanol and (LD10 =39.17 mg/kg, LD50 =110.3 mg/kg) in ethyl acetate respectively. The fungi isolated from seed of Azadirachta indica were (LD10 =12.25 mg/kg, LD50 =39.80 mg/kg) in methanol and (LD10 =28.97 mg/kg, LD50 =60.20 mg/kg) in ethyl acetate respectively. The mortality increases with increase in concentration of endophytic fungi. The methanol solvent extract showed more insecticidal property against Callosobruchus chinensis due to the secondary metabolites of endophytic fungi. Statistical variance, 95% confidence limits and regression equations are presented.

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