The Bio-diversity have been declining within the arid part of Rajasthan over the last few decades. The decrease in the population of wild fauna may be also due to destruction of natural habitats like illegal sand mining, stone quarries, increasing feral dog population and poaching. Guda Bishnoi area is major habitats for large number of terrestrial animals and birds in the Jodhpur district of Rajasthan. Here the Luni river bed has diversity and populations of wild fauna. These wild animals use this river banks for grazing, resting, moving and breeding. Simultaneously, these river beds are also used for sand mining purposes by people. The wide use of sand mining on the habitat of wild animals has a directly impact on their population and diversity. We studied the mining impact on bio-diversity of Guda Bishnoi area of Jodhpur district, Rajasthan during 2003-2006 and 2012-13 is discussed in this paper.