High affinity of the fluoride to the bone causes intoxication of bone marrow resulting into alterations in normal hematological profile. In present study sodium fluoride alone and with Vitamin E (ameliorative agent) was administered orally daily for 45th days. For this purpose, a total of 24 rats were randomly divided into four equal groups: group I used as control and groups II, III and IV were orally treated with Sodium fluoride (8 mg/kg body weight), Vitamin E (100 mg/kg body weight) and Sodium fluoride plus Vitamin E, respectively to access the effect on the hematological profile on different days of exposure. Exposure of sodium fluoride alone produced significant reduction in hemoglobin (Hb), packed cell volume (PCV), total leucocytes count (TLC), and total erythrocyte counts (TEC) and means corpuscular volume (MCV). However, values of mean corpuscular hemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC) were not significantly changed. On comparing the observations of ameliorative group with the toxicity group rats, it is concluded that the use of the ameliorative Vitamin E has beneficial effects on the alterations in values of Hb, PCV, TEC, TLC and MCV produced by the subacute exposure of Sodium fluoride in rats.