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Year: 2016, Issue: December
Can nitrogen limitation produce any dietary response in the marine microalga Chlorella marina - A combo diet in green water hatchery.
Rajani S. Ganga and Aneykutty Joseph
Chlorella marina, Growth kinetics, Biochemical variations, Nitrogen limitation.
The present study was conducted to assess the influence of nitrogen on culture media on the growth response as well as biochemical composition of the marine microalga, Chlorella marina. Comparative growth and multiplication of Chlorella marina was studied using 5 different nutrient conditions viz. Walnes medium (control/N ), 100 Walnes medium without nitrogen source (N ) and Walnes medium with 25% (N ), 50% (N ) and 75% (N ) nitrogen 0 25 50 75 sources. Cultures were maintained in Haffkine flasks (4 liters) in triplicates. Standard algal culture conditions viz. medium, in sterilized sea water of salinity 33 ppt, standard pH of 8.5, culture room temperature of 24�C and light intensity of 3000 lux (12hr:12hr). Algal cell density was estimated using a haemocytometer on every alternate days. Growth kinetics and biochemical variations of Chlorella marina in each culture condition were analysed following standard methods. One way analysis of data revealed that nitrogen limitation is found to influence cell division rate and biochemical compositions of Chlorella marina significantly (p
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