An attempt has been made to induce spawning and larval rearing of the climbing perch, A.testudineus, under agro-climatic conditions of Raipur (C.G.). Male and female brooders were identified based on secondary sexual characters - the males being darker in colour with oozing milt by applying slight pressure on belly while females possessed light brown pigmented spot on body with bulging abdomen. Females were given intraperitoneal (i.p.) injection of ovatide @ 0.06 ml/100 g body weight while males were administered (i.p.) with the hormonal drug @ 0.04 ml/100 g weight. The injected brood fish sets comprising one female and two males (sex ratio 2:1) were released in cement cistern. Breeding was observed in all the 3 sets of climbing perch but the latency period was prolonged to 18-28 hours. Eggs (released in batches) were very minute and floating on the surface of water. The diameter of fertilized eggs ranged between 0.6-0.7 mm and were bright clear and transparent while unfertilized eggs appeared milky and opaque. The average fertilization rate was 90% under the hatchery conditions indicating successful natural spawning (without stripping). The newly-hatched larvae with yolk sac measured 2.0-2.3 mm on day one, 2.6-2.8 mm on day two and 3.0-3.6 mm on day three (after absorption of yolk sac). Air-breathing organ developed on 11th day and larvae measured 10.6- 11.8 mm. Indoor rearing of the larvae was done on feed such as plankton, egg custard and chopped molluscan meat with water depth of 6-8". From the three sets of brooders, 10,600 hatchlings and ultimately 4,686 fry were produced.