The pollution load of organic/inorganic nature in these drains discharging effluent in river Ganga in the stretch between Farrukhabad to Varanasi, is being measured in terms of Biochemical oxygen demand (BOD), Chemical oxygen demand (COD), different ions and bacteria. Assessment of biodegradability of wastewater is performed by the ratio of BOD:COD. In this study, total thirty five major drains meeting to river Ganga directly or indirectly between Farrukhabad to Varanasi, were consider for assessing the degree of their biodegradability. BOD:COD of different drains were found ranging 0.23-0.33, 0.31-0.45, 0.25-0.5, 0.35, 0.19-0.7, 0.32-0.4 and 0.38-0.46 at Farrukhabad, Kanpur, Unnao, Raebareli, Allahabad, Mirzapur and Varanasi respectively. Lower ratio indicated non-biodegradability of the wastewater. Study of physico-chemical characteristics, biodegradability index and pollution load helps planning the treatment strategies of wastewater. Based on the BOD:COD calculated for different drains, biodegradability of drain can be categorised as good - >0.5 (4 drains), moderate 0.3-0.5 (22 drains) and poor <0.3 (9 drains).