The present study is carried out to detect and isolate the entomoparatic nematodes from Periplaneta americana. Cockroaches were collected from different residential areas of Aurangabad city, identified and dissected. Nematode parasites are isolated from gut of cockroaches.70 cockroaches were dissected, 77% cockroaches were infected. Nematodes found Hammerschimidtiella diesingi, larva, adult females, eggs, Leidynema appendiculata, Thelastoma bulhoesi. This study shows that cockroaches are potenial carrier of nematodes parasites since these parasites can transferred t Aurangabad o humans.