Pesticides are the chemical compounds widely used on the variety of crops to control the pests for improvement of food production. Monocrotophos is one of the most commonly used organophosphate insecticides in the developing countries like India. The present study investigates the cytotoxicity induced after 24 hours of monocrotophos exposure in the intestine of the Swiss albino male mice. Intestine being the absorptive area of the body and due to its large surface area seems to be the primary site of the exposure for the nutrients and the different xenobiotics including pesticides. Monocrotophos was administered orally in mice at the doses of 1.25 mg/kg, 2.5 mg/kg and 5.0 mg/kg. Many histopathological changes such as degeneration of epithelium and lamina propria, distortion in crypts, degeneration of submucosa with congested, enlarged and folded villi were noticed in the small intestine of the mice in a dose dependent manner. The results of the study showed that monocrotophos have deleterious effects on the intestine of Swiss albino mice.