An attempt has been made to assess the alteration in hematological parameters in albino rat on exposure to air pollution in three different studied regions viz.; Kothari Market (Commercial), Sanver Road (Industrial) and Vijayanagar (Residential) of Indore city during May-June and July-August (2014). The Hemoglobin (g/dl), RBCs count (x106/?L) and Blood platelets (x103/?L) was found to be decreases (P<0.01%) whereas, increased values (P<0.01%) of WBCs count (x103/?L) were noticed in all these three different studied regions of Indore city during May-June and July-August (2014). The results of present investigation were expressed as mean and standard deviation and; the significance level between the means of control and the experimental groups were checked by Student's t-test. Results indicate that pollution level elevated in Indore city which may produce adverse effect on human life.