Biological control of anthracnose disease of Saraca asoca caused by Colletotrichum gloeosporioides was made with the application of antagonistic agents like Trichoderma viride, T.harzianum, T.lignorum, T.hamatum and T.koningii. The effect of volatile and non-volatile antibiotics of Trichoderma origin on growth inhibition of the anthracnose pathogen was studied T.harzianum showed maximum growth inhibition of the pathogen through mycoparasitism and the non-volatiles produced by the same agent exhibited its excellent antagonism to the growth of the pathogen (100%) under in vitro condition and that the effect was also proved to be durable.