Tapti river is one of the major rivers of India, with the total length of around 724 kilometres starting from Betul (M.P.) to Surat (Gujrat). In the present study the ichthyofaunal diversity of river Tapti in Burhanpur district was undertaken from December,2014 to August,2015.The present study showed 26 fish species belonging to 7 families, 6 orders were recorded in the 4 respective stations viz. Burhanpur (Station-1), Bhatkheda (Station-2), Dariyapur kalan (Station-3), Jainabad (Station-4). The total 26 fish species recorded at Station-1 (Burhanpur), 25 fish species recorded at Station-2 (Bhatkheda), 24 fish species recorded at Station-3 (Dariyapur kalan) 26 fish species recorded at Station-4 (Jainabad). The member of order Cypriniformes were dominated by 13 fish species followed by Siluriformes 6 species, Ophiocephaliformes 3 species, Mastacembeliformes 2 species, Clupeiforme 1 species, Beloniformes 1 species.