The present study deals with floristic composition, life forms and soil analysis of the constituent species of sand dune ecosystem of Rajasthan desert region. During favorable season vegetation consists of many ephemerals, annuals and perennials and maximum number of species was recorded during rainy season. The sand dune flora is predominantly therophytic. Maximum numbers of the species are present on base and leeward side of the sand dune. Least number of species is present on the top. Soil sample were taken from different depth of top, slope and base of sand dune during summer, winter and rainy season and analyzed for pH, Electrical Conductivity, Organic Carbon, Available Phosphorus, Available Potassium, Zinc, Iron, Copper and Manganese. Significant variations were observed in texture as well as physico-chemical characteristic of soil at different level. Plants of top, slope and base also showed variations in species composition and characteristic habit.