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Year: 2016, Issue: December
Phytochemical screening and in vitro antioxidant activities of chloroform soluble fraction of Simarouba glauca leaf extract.
Anu Gopalakrishnan, Usha, P. T. A., Bibu John Kariyil, Reni John and Surya Sunilkumar
Simarouba glauca, Chloroform soluble fraction, Phytochemicals, Antioxidant
Simarouba glauca leaves and bark are being used traditionally for the treatment of several diseases. In the present study chloroform soluble fraction (CSF) separated from the ethanolic extract of Simarouba glauca leaves has been investigated for the presence of various phytochemicals. The in vitro antioxidant potential of the fraction was also assessed by means of superoxide and nitric oxide radical scavenging assays. Phytochemical screening revealed the presence of steroids, alkaloids, flavonoids, glycosides, diterpenes and triterpenes whereas saponins and tannins were absent. CSF of Simarouba glauca extract exhibited marked scavenging activity against superoxide anion radical (O .-) 2 and nitric oxide radical (NO) in a dose dependent manner comparable to the effect produced by vitamin C standard. The EC value for superoxide radical inhibition by CSF of Simarouba glauca was 35.12�4.02 ?g/ml and for nitric oxide 50 radical inhibition EC obtained was 46.05�7.09. Hence the present study identified that chloroform soluble fraction of 50 Simarouba glauca leaf extract is rich in potent phytochemicals and it possesses marked antioxidant activity in a dose dependent manner.
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