The present study was conducted to examine the variation in total protein and glucose profile of the urine during early pregnancy in buffaloes. Urine samples were obtained from 30 buffaloes (15 pregnant and 15 non pregnant) and assayed for total protein and glucose. Results indicated that total protein and glucose concentration of urine varied significantly between pregnant and non-pregnant group. Glucose and protein concentrations in pregnant group showed significantly difference from non-pregnant group from day 14 to 30 of estrous cycle.