Year: 2019, Issue: December
Improving GLYCINE MAX L. water stress tolerance targeting superoxide dismutase
Meenakshi Mishra, Vijaylakshmi Jain, Pankaj K. Mishra and Veeru Prakash
Waterlogging stress, HPLC, Reactive oxygen species, Salicylic acid, Superoxide dismutase.
Superoxide dismutase (SOD) is an important anti-oxidative enzyme by means of various therapeutic applications. Present investigation dealt with characterization of copper-zinc SOD (CuZn-SOD) from Glycine max L. grown in waterlogging stress in the midst of salicylic acid (SA). Ammonium sulfate fractionation and HPLC results set the presence of CuZn-SOD in the extract under applied stress which enhanced SOD activity 3-5 folds with the total yield 63-65% after partial purification. The purified protein was characterized as CuZn-SOD, reflected by H O and 2 2 NaN induced inhibition. The optimum pH range for purified CuZn-SOD activity was reported 7.5 and this was stable 3 over a wide range of temperatures 20-50ºC. This is a homodimeric protein with subunit molecular weight of 33.2 kDa. The peak area and height in HPLC were recorded from 448525-13987576 and 83628-955538 respectively from T -T . 1 0 Outcome also showed the shielding effects of SA against hypoxic conditions.