Year: 2019, Issue: December

Improving GLYCINE MAX L. water stress tolerance targeting superoxide dismutase

Author:

Meenakshi Mishra, Vijaylakshmi Jain, Pankaj K. Mishra and Veeru Prakash

Keyword:

Waterlogging stress, HPLC, Reactive oxygen species, Salicylic acid, Superoxide dismutase.

Superoxide dismutase (SOD) is an important anti-oxidative enzyme by means of various therapeutic applications. Present investigation dealt with characterization of copper-zinc SOD (CuZn-SOD) from Glycine max L. grown in waterlogging stress in the midst of salicylic acid (SA). Ammonium sulfate fractionation and HPLC results set the presence of CuZn-SOD in the extract under applied stress which enhanced SOD activity 3-5 folds with the total yield 63-65% after partial purification. The purified protein was characterized as CuZn-SOD, reflected by H O and 2 2 NaN induced inhibition. The optimum pH range for purified CuZn-SOD activity was reported 7.5 and this was stable 3 over a wide range of temperatures 20-50ºC. This is a homodimeric protein with subunit molecular weight of 33.2 kDa. The peak area and height in HPLC were recorded from 448525-13987576 and 83628-955538 respectively from T -T . 1 0 Outcome also showed the shielding effects of SA against hypoxic conditions.

Society of Life Sciences

LSB
e - ISSN: 2321-7952
Print ISSN: 0973-5453X
News
Subscriptions
Hard Copy Subscription
Coming Issues
Categories
Animal Science
Agricultural Science
Biotecnology
Microbiology and Virology
Environment
Medical and Forensics
Plant Science
Anatomy and Physiology
Biology
Neuroscience
Ecology
Zoology
1/25
Website Visit Counter
NJLS
e - ISSN: 2321-7960
Print ISSN: 0972-995X