The paper deals with the metal contents in water, sediment, macrophytes, plankton (including both Phytoplankton and zooplankton), macroinvertebrates and fish (herbivorous and carnivorous) of Gandhisagar reservoir, Mandsour district, Madhya Pradesh. The samples were acid digested and subjected to AAS analysis for Hg, Pb and Cd. The results showed that the selected heavy metals in water, sediment, macrophytes, plankton, macroinvertibrates (benthos) and fish are distinguishable with sediment and biota samples are more susceptible to metal accumulation. It is assumed that the Pb and Cd concentration values in water, sediments, macrophytes, plankton, macroinvertibrates and fish were very low or below the safe concentration ranges for natural waters suggested by FAO. The Hg levels detected reveals that out of the three metals considered in the present study, Hg accumulation is higher in all the food fishes studied followed by Pb and Cd.