An investigation was carried out to study the efficacy of different bio-agents (Trichoderma viride and neem cake) and chemical nematicide triazophos, on mungbean (Vigna radiata L. Wilczek) susceptible cultivar PDM1 infected with root-knot nematode, Meloidogyne incognita race 3. Results revealed that seed treatment of mungbean seed with Trichoderma viride concentration @ 6 g/kg and @ 3 g/kg significantly increased the root length, shoot length, number of pods, number of Rhizobium leguminosarum nodules, fresh and dry weight of shoot and yield per plant of mungbean, followed by treatment of mungbean seed with neem seed powder at concentration @ 5% w/w and @ 3% w/w further followed by seed treatment with triazophos 40 EC at concentration @ 2% w/w and 1% w/w as compared to untreated control. Among the different bio-agents and chemical nematicide, seed treatment of mungbean seed cultivar PDM1, with Trichoderma viride at concentration @ 6 g/kg and @ 3 g/kg exhibited the best result in reducing the fresh and dry weight of root, number of galls per root system, egg masses per root system and initial and final total nematode population followed by neem seed powder in concentration @ 5% w/w and @ 3% w/w. Application of triazophos 40 EC @ 2% w/w and 1% w/w as seed treatment chemical nematicide was least effective. However all the 6 treatments were effective in controlling the menace of root-knot nematode, Meloidogyne incognita race 3.