The application of commercially available fish meal is the most expensive protein source in diets for fish. In order to reduce the cost of feed locally available by-catch fish wastes is used as protein supplement. The noncommercial, poor quality by catch trash fish like puntius, glassfish and molluscs are selected as protein supplement ingredients in formulating low cost nutritional feed for Labeo rohita. These fish were collected, dried, powdered and analyzed for their proximate composition such as moisture, crude protein, crude fat, crude fibre and nitrogen free extract.