The immunomodulatory effect of Bacopa monnieri (Brahmi) in enhancing stress tolerance in Labeo rohita (Hamilton,1822) has been investigated. The present study has been designed to examine the specific activity of the antioxidant enzymes, especially Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) after feeding Labeo rohita with Brahmi incorporated feed. The results showed significant variation in the specific activity of antioxidant enzymes before and after the exposure to the stress. In this maximum variation occur in the antioxidant enzymes, Superoxide dismutase activity is seen in the feed having 15% Brahmi and values ranged from 1.323-2.284. This result is near to the result got for 20% and the value varies from 0.974-2.275. The minimum variation occur in the feed contain 5% Brahmi and values varied from 0.552-0.929. In the Glutathione peroxidase maximum variation occurs in the feed contain 15% Brahmi and values ranged from 0.109-0.690. The minimum variation occurs in control feed and values come from 0.039-0.153. Comparison of the specific activities of the antioxidant enzymes before and after stress exposure indicated that incorporation of 15% Brahmi in diet is the optimum level to enhance stress tolerance in L.rohita.