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Year: 2016, Issue: December
Plant regeneration via somatic embryogenesis from the cultured immature male floral buds of banana genotype 'Dwarf Cavendish'.
Sugandh Suman, Manish Kumar and Harsh Kumar
Banana, Micropropagation, Dwarf cavendish, Immature male floral bud.
Immature male floral buds of banana cv. Dwarf Cavendish were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of 2,4-D (4.52, 9.05 and 18.10 ?M), IAA (5.71, 11.42 and 22.83 ?M), Kin (4.65, 9.30 and 18.60 ?M) and BAP (4.43, 8.87 and 17.74 ?M). The culture of the explants resulted in the generation of small, spherical and friable calli. These calli showed embryogenic characteristics and sub-cultured on the same medium for further maturation. After 5 months of culture on MS basal medium, somatic embryos started to regenerate. The somatic embryos were transferred to modified MS medium for regeneration into plantlets. Among the different combinations tried, MS +9.05 ?M 2,4-D + 18.60 ?M Kin was found to be the most suitable medium for somatic embryogenesis. Thus, an efficient protocol for plant regeneration via indirect somatic embryogenesis has been developed.
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