The present study was conducted for assessment of oxidative stress and antioxidant activities for amelioration of diabetes, were evaluated in vivo, in wistar rats. In vivo, oxidant/antioxidant status revealed that oxidative stress play a pivotal role in pathogenesis of diabetes. SOD and GSH levels, were found significantly low in diabetic rats compared with healthy (negative control) animals. LPO and Catalase were found significantly higher in diabetic rats. Antioxidant potential of Vitamin C, Vitamin E, Spirulina and Madhuca bark extract were evaluated to know the best antioxidants among them. Spirulina exerted the most erudite antioxidant with antidiabetic activity, followed by Madhuca bark extract. Spirulina showed an obvious recovery approximately close to normal status in experimental diabetic rats. The finding of the present study clearly indicated that, Spirulina can be used as an alternative natural antioxidant as well as powerful hypoglycemic agent in diabetes management and might therefore be helpful for treating diabetes and related complications.