Chlorpyrifos is a broad-spectrum, moderately toxic insecticide that has been widely used in the prevention of agricultural pests. In the present study seven chlorpyrifos pesticide utilizing bacteria were isolated and identified through cultural and biochemical tests as strains of Bacillus sps., Staphylococcus sps., Coccus sps., Bacillus sps. was identified as Bacillus cereus by 16 srRNA analysis. Their growth in minimal salt medium supplemented with 200 µg/ml and 250 µg/ml of Chlorpyrifos was monitored at optical density of 600 nm. The result showed that Bacillus cereus had maximum growth at fourth days. The results of this research indicated that the isolated bacteria can be used for bioremediation of Chlorpyrifos contaminated soil.