Phenol is one of the most common pollutants present in the industrial waste. It create many health problem in human as well as in many other animals. In present study three different samples were collected from the Dada Nagar industrial area Kanpur, from these sample we have isolated 15 different bacteria. These bacteria were subjected for the phenol tolerance test. From these bacteria we were isolated four bacteria (DI-2, DI-3, SWD-2, DFS-2) who have good level of phenol tolerance. All four bacteria were subjected for comparative phenol degradation study. We found that all four bacteria have the capacity to degrade phenol but the most efficient bacteria for phenol degrading was DFS-2. These four bacteria were identified as DI-2 (Pseudomonas aeruginosa), DI-3 (Pseudomonas citronellolis), SWD-2 (Klebsiella spp.,), SDF-2 (Pseudomonas putida).