Bori river is seasonal tributary near Shirud village of Dhule. The present icthyofaunal study was done from June,2013-May,2015, for three season's i.e. Monsoon, winter and summer. During the study period altogether 17 fish species belonging 4 families and 5 orders were found in the river. Cypriniformes was the major order had 9 species then by species, Siluriformes 3 species, Synbranchiformes 2 species, Channiformis 2 species and Osteoglossifomes with 1 species.