This study is intended to provide the mass rearing and laboratory method for the establishment of Zygograma bicolorata of family Chrysomelidae, also referred as Mexican beetle. These were introduced for biological control on noxious weed called Parthenium hysterophorus so are also referred as 'Parthenium beetle'. The life cycle of the beetle is of approximately 4-6 weeks which is divided into four different stages : Egg, Larvae, Pupa and Adult. The larval stage comprises of four instars. The adult feeds on fresh and young leaves of parthenium and tends to hide on adaxial side of the leaves. An effort has been made for mass culturing the beetles in laboratory conditions.