Year: 2016, Issue: June

Identification and antibiotic sensitivity of Staphylococcus spp. in sub-clinical mastitis in cattle.
Author:
Pawan Maheshwari, P. C. Shukla, Jyoti Dongre and Jitendra Shivhare
Keyword:
Mastitis, MCMT, Staphylococcus spp., Enrofloxacin
Mastitis, caused by multi factorial etiopathological factors, is one of the costliest disease of dairy animals across the globe. The study was conducted for a period of more than 1 year i.e. from February,2015 to February,2016. For the epidemiological study 550 lactating cows were screened by modified California mastitis test (MCMT). Clinical examination of animals and their udder/milk and phenotypic characterization of Staphylococcus spp. was carried out. The overall occurrence of Staphylococcal SCM was reported to be 16.36% (90/550) animal wise and 6.07% (127/2092) quarter wise as identified on the basis of colony morphology, characteristic colour changes on Mannitol salt agar, positive catalase test, haemolysis pattern on blood agar and DNase activity on DNase agar. Results of antibiogram revealed that most effective antimicrobial agent was enrofloxacin (100%) followed by piperacillin + tazobactum (87.80%), cefoparazone (82.92%), ampicillin + sulbactum (82.92%) and cefuroxime (82.92%) against Staphylococcus spp. All the isolates showed resistance to cefepime (100%).

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