Present paper deals with the study of Spermatogenesis of a new digenetic trematode Allocreadium ophiocephali, Srivastava,1960. The helminthes parasites were collected from the intestine of a fresh water fish Ophiocephalus punctatus of the family Allocreadiidae stossich, at the locality Dhirma Nala, Rewa (M.P.) in the month of October and November. A few of the parasites were flattened on a clean slide under the slight pressure of a cover glass and fixed in alcoholic Bouin's fluid for 12 hours. Stains like Gower's Carmine, Mayer's paracarmine and Haemalum were used for the preparation of whole mounts for identification and the study of testes and process of Spermatogenesis in this specie.